Reconstitution of Active Mycobacterial Binuclear Iron Monooxygenase Complex in Escherichia coli

Reconstitution of the Escherichia coli pyruvate dehydrogenase complex.

The binding of pyruvate dehydrogenase and dihydrolipoyl dehydrogenase (flavoprotein) to dihydrolipoyl transacetylase, the core enzyme of the E. coli pyruvate dehydrogenase complex [EC 1.2.4.1:pyruvate:lipoate oxidoreductase (decaryboxylating and acceptor-acetylating)], has been studied using sedimentation equilibrium analysis and radioactive enzymes in conjunction with gel filtration chromatogr...

Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in Escherichia coli.

The hOCTN1 (human organic cation transporter 1) overexpressed in Escherichia coli and purified by Ni-chelating chromatography has been reconstituted in liposomes by detergent removal with a batch-wise procedure. The reconstitution was optimized with respect to the protein concentration, the detergent/phospholipid ratio and the time of incubation with Amberlite XAD-4 resin. Time-dependent [(14)C...

Escherichia coli . pentachlorophenol - 4 - monooxygenase gene in the Flavobacterium Cloning

Molecular cloning of mycobacterial promoters in Escherichia coli.

Mycobacterium bovis BCG chromosomal DNA, digested with EcoR1 and HindIII, was used to construct a promoter library in Escherichia coli using the promoter probe plasmid pKO-1. DNA inserts of various sizes showed promoter activity judged by the level of galactokinase (galK) whose synthesis they activate (between 50 and 850 galactokinase units). No correlation between the length of the DNA insert ...

Iron uptake by Escherichia coli.

Ferric iron is transported into Escherichia coli by a number of chelating compounds. Iron transport through the outer membrane by citrate, ferrichrome, enterobactin, aerobactin, yersiniabactin, and heme is catalyzed by highly specific proteins and across the cytoplasmic membrane by ABC transport systems with lower specificity. Transport across the outer membrane requires energy, which is provid...